Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Amoussou-Guénou, K.M.a; * | Squitiero, B.a | Voutay, M.a | Labat, B.b | Rusch, Ph.c
Affiliations: [a] Laboratoire de Biophysique, Faculté de Médecine, Saint-Etienne, France | [b] Laboratoire de Biochimie, Faculté de Médecine, Saint-Etienne, France | [c] Laboratoire d’Informatique Médicale, Faculté de Médecine, Saint-Etienne, France
Correspondence: [*] K.M. Amoussou-Guénou: Universitetet I Oslo, Fysisk institutt, Post boks 1048, Blindern, 0316 Oslo, Norway. Tel.: +47 22 85 64 32; fax: +47 22 85 64 22; e-mail: [email protected].
Abstract: The purpose of this study was to identify a parameter allowing the standardization of the Cell Transit Analyzer (CTA) in order to study the individual deformability of each explored Red Blood Cell (RBC). Using theoretical arguments based on the principle of the CTA, we calculated the thickness “x” of the crown of fluid surrounding each RBC during its entry phase into the micropore. A mathematical equation (x=62.5/|dU|) was established between the difference of potential (U, mV) that occurs during this phase and the corresponding thickness (“x”, μm) of the crown. By exploring fresh control RBCs of healthy subjects and assuming that the rigid RBCs proportion in a fresh blood sample of healthy subject is less than 3.5%, we performed a thresholding of “x” to distinguish rigid RBC from deformable ones. That thresholding was necessary to stamp the variability of counts linked to polycarbonate filters (PF) used to carry out measures. According to the PF, the value of the threshold “Tx” provided by the thresholding ranged between 0.222 and 0.246 μm. Using the values of “Tx”, we counted approximately 10–25% rigid RBCs in the pathological samples of four patients with sickle cells SS disease and of one diabetic patient with splenomegaly. We observed in addition that the percentages of rigid RBCs counted after thresholding are identical from a filter to another one with an absolute error less than 2% in fresh RBCs (normal or pathological) samples. We concluded that the method of standardization by thresholding presented here could be used in clinic routine to count the rigid RBCs percentage contained in a given sample.
Keywords: CTA, standardization, cellular deformability, RBCs, rigid subpopulation
DOI: 10.3233/THC-1997-5502
Journal: Technology and Health Care, vol. 5, no. 5, pp. 347-357, 1997
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
[email protected]
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office [email protected]
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
如果您在出版方面需要帮助或有任何建, 件至: [email protected]