Affiliations: [a] Department of Chemistry, Government College University, Faisalabad 38000, Pakistan. e-mail: [email protected]
| [b] Department of Allied Health Sciences and Chemical Pathology, University of Health Sciences, Lahore 54000, Pakistan | [c] Institute of Chemistry, University of the Punjab, Lahore 54000, Pakistan | [d] Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan | [e] Interdisciplinary Research Centre in Biomedical Materials, COMSATS Institute of Information Technology, Lahore 54000, Pakistan
Abstract: Biological evaluation of honey bee hive extracts was estimated using a set of chemical and biological in vitro models. Extractions of bee hives were done in methanol and acetone solvents. Well-tolerated yield was observed in case of acetone solvent (12.34 ± 1.28 %) and methanol solvent (5.98 ± 0.94 %). Total phenolic and total flavonoid contents were found in the range of 359 ± 4.06–755 ± 6.03 and 0.952 ± 0.08–1.266 ± 0.04 mg/100 g dry extract, respectively. Antioxidant potential was tested using most reliable in vitro models, such as DPPH free radical scavenging model, lipid peroxidation inhibition in linoleic acid system and ABTS radical scavenging assay. The DPPH scavenging assay showed 28–55 % radical scavenging, while the peroxidation inhibition in linoleic acid system showed low peroxidation inhibition 6–26 %. TEAC value showed 4.12 ± 0.19 and 3.63 ± 0.5 μM TE/g for methanol and acetone extract, respectively. The antibacterial assay of methanol and acetone extracts showed good zone of inhibition in following order S. aureus (19.1 ± 1.15, 14.9 ± 0.53 mm) >B. subtilis (15.4 ± 0.07, 13.1 ± 0.72 mm) >E. coli (13.8 ± 0.95, 9.9 ± 0.35 mm). Overall result demonstrated promising antioxidant and antibacterial potential of honey bee hive extracts.
