Affiliations: Fujian Provincial Key Laboratory of Photonic
Technology, Key Laboratory of Optoelectronic Science and Technology for
Medicine, College of Photonic and Electronic Engineering, Fujian Normal
University, Ministry of Education, Fuzhou, Fujian, China | Department of Cardiovascular Surgery, Fujian
Provincial Hospital, Fuzhou, Fujian, China | Biophotonics Research Laboratory Department of
Engineering and Physics, University of Central Oklahoma, Edmond, OK, USA
Note: [] Corresponding authors: Hui Li, Fujian Provincial Key Laboratory
of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology
for Medicine, College of Photonic and Electronic Engineering, Fujian Normal
University, Ministry of Education, Fuzhou 350007, Fujian, China. Tel.: +86 591
2286 8133; E-mail: [email protected]. Wei R. Chen, Biophotonics Research
Laboratory Department of Engineering and Physics, University of Central
Oklahoma, Edmond, OK 73034, USA. Tel.: +1 405 974 5147; E-mail: [email protected]
Abstract: A method combining morphological granulometry with Mie theory to
determine optical scattering in biological tissues was proposed. Otsu's method
was applied to binarize phase-contrast images. Binary morphological
granulometry was used to estimate size density distribution of the tissue
samples based on the binary phase-contrast images. Our results showed that the
optical parameters associated with light scattering in tissue could be
quantitatively determined by combining size density distribution with Mie
theory. It was suggested that this unique method could be used to characterize
biological tissues for disease diagnosis.
Keywords: Morphological granulometry, optical scattering properties, Mie theory
