A novel ^{99m}Tc hyaluronate probe for SPECT imaging of lung cancer - feasibility study in an animal model

Article type: Research Article

Authors: Qiang, Yong Gang | Liao, Yong Hua | Li, Jian | Zhang, Xiu Ping | Huang, Zheng

Affiliations: Guangzhou Medical College, Guangzhou 510182, PR China | Guangzhou Cancer Hospital, Guangzhou 510095, PR China

Note: [] Corresponding author: Department of Experiment Nuclear Medicine, Guangzhou Medical College, Guangzhou 510182, P.R. China. Tel.: +86 20 81340333; E-mail: [email protected]

Abstract: This study described a novel radioactive probe for SPECT imaging of lung cancer. The probe was consisted of ^{99m}Tc labeled cartilage link protein (CLP). The CLP, a hyaluronate binding protein, was isolated from bovine nose cartilage and purified on a chromatography column. ^{99m}Tc labeling was carried out by pre-treating CLP with SnCl_2. The feasibility of this probe for lung cancer imaging was tested in vivo in an animal model. The hyaluronate probe showed a fairly good binding activity towards both natural and tumor hyaluronic acid in in vitro and in vivo assays. The preliminary feasibility study in a human lung cancer bearing mouse model showed that the probe might be useful for tumor imaging.

Keywords: hyaluronate probe, cartilage link protein, SPECT, lung cancer

Journal: Journal of X-Ray Science and Technology, vol. 13, no. 2, pp. 97-106, 2005