Affiliations: Department of Clinical Sciences, Oregon State University College of Veterinary Medicine, Corvallis, OR, USA | Department of Pediatrics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA | Department of Pathobiological Sciences, University of Wisconsin-Madison School of Veterinary Medicine, Madison, WI, USA
Note: [] Corresponding author: Dr. Keith P. Poulsen, Department of Clinical Sciences, Oregon State University College of Veterinary Medicine, 700 SW 30th Street, Corvallis, OR 97331 USA. Tel.: +1 541 737 6939; Fax: +1 541 737 8651; E-mail: [email protected]
Abstract: The objectives of this study were to develop an animal model to study Listeria monocytogenes infection during the peri-parturient period and identify sources of maternal shedding of the pathogen. Peri-parturient mice were infected intragastrically with L. monocytogenes that expressed bacterial luciferase. Mice were then imaged in vivo over time. Secreted breast milk samples from mice infected after parturition were enriched and plated for culture and imaging. Bioluminescence imaging technology was able to detect luciferase emitting L. monocytogenes in vaginal secretions and maternal and fetal organs at 72 and 96 h post infection in mice infected prior to, or just after, parturition. The results from this study clearly show that L. monocytogenes is shed in vaginal secretions and disseminates to the mammary chain, from which it can be shed in the milk of peri-parturient mice.
Keywords: Listeria monocytogenes, breast milk, bioluminescence
