Affiliations: [a] Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA
| [b] Universidad de San Martin de Porres, Facultad de Medicina Humana, Instituto de Investigación, Lima, Peru
| [c] Asociación Civil PROESA, Lima, Peru
| [d] Department of Obstetrics and Gynecology, University of Utah Health, Salt Lake City, UT, USA
| [e] Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA
| [f] The Chester M. Pierce, M.D. Division of Global Psychiatry, Massachusetts General Hospital, and Harvard Medical School, Boston, MA, USA
Correspondence:
[*]
Address for correspondence: Diana L. Juvinao-Quintero, PhD, Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, MA 02115. Tel.: +1 774 315 6347; E-mail: [email protected].
Abstract: BACKGROUND:Preterm birth (PTB) affects ∼15 million pregnancies worldwide. Genetic studies have identified several candidate loci for PTB, but results remain inconclusive and limited to European populations. Thus, we conducted a genome-wide association study (GWAS) of PTB and gestational age at delivery (GA) among 2,212 Peruvian women. METHODS:PTB cases delivered≥20 weeks’ but < 37 weeks’ gestation, while controls delivered at term (≥37 weeks but <42 weeks). Multivariable regressions were used to identify genetic markers for PTB and GA (∼6 million SNPs), adjusting for maternal age and the first two genetic principal components. In silico functional analysis was conducted among top signals detected with an arbitrary P < 1.0×10–5 . We sought to replicate genetic markers for PTB and GA identified in Europeans, and we developed a genetic risk score for GA based on European markers. RESULTS:Mean GA was 30 ± 4 weeks in PTB cases (N = 933) and 39 ± 1 in the controls (N = 1,279). No associatiosn were identified at genome-wide level. Nominal PTB variants were enriched for biological pathways associated with polyketide, progesterone, steroid hormones, and glycosyl metabolism. Nominal GA variants were enriched in intronic regions and cancer pathways. Variants in WNT4 associated with GA in Europeans were replicated in our study. A genetic risk score was associated with a 2-day longer GA (P = 0.002) in our sample. CONCLUSIONS:This study identified various signals suggestively associated with PTB and GA in pregnant Peruvian women. None of these variants overlapped with signals previously identified in Europeans.
Keywords: Genetic risk score, genome-wide association study, gestational age at delivery, pregnancy, preterm birth
