Affiliations: Department of Obstetrics and Gynecology, Akershus
University Hospital, Lørenskog, Norway | Institute of Clinical Epidemiology and Molecular
Biology, Akershus University Hospital, Lørenskog, Norway | Department of Pathology, Faculty Division Akershus
University Hospital, University of Oslo, N-1478 Lørenskog, Norway
Note: [] Corresponding author: Berit Dahlstrøm, Department of
Obstetrics and Gynecology, Akershus University Hospital, P.B.24 Ahus, N-1478
Lørenskog, Norway. Tel.: +47 91694411; E-mail: [email protected]
Abstract: The aim of this study was to develop an assay method for
determination of down stream gene expression in endothelial cells from human
decidual tissue preserved in an RNA-conserving buffer. Human decidual tissue
was collected and preserved in RNAlater. The tissue samples were dissected and
passed through a mechanical tissue disaggregator, incubated with CD31-coated
paramagnetic beads and the attached cells were extracted. RNA isolated from the
enriched cells proved to be conserved with good quality and a genome
RNA-microarray was successfully performed. The method provides an alternative
for studying in vivo gene expression in endothelial cells from human
decidual tissue.
Keywords: HEEC, endothelial cells, in vivo gene expression, cell isolation, RNA degradation
