Affiliations: [a]
Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA
| [b]
Department of Medicine and The RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA
| [c]
State Key Laboratory of Medical Neurobiology, School of Life Sciences, Fudan University, China
| [d]
Department of Neurology, Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA
Correspondence:
[*]
Correspondence to: Marian DiFiglia, Ph.D., MassGeneral Institute for Neurodegeneration (MIND), Department of Neurology, Massachusetts General Hospital, 114 16th Street, Room 2002, Charlestown, MA 02129, USA. Tel.: +1 617 726 8446; Fax: +1 617 726 1264; E-mail: [email protected].
Abstract: Background: Reducing mutant huntingtin (mHTT) in neurons may be a therapy for Huntington’s disease (HD). Elevating NUB1 protein reduced mHTT levels in cell and fly models of HD through a proteasome dependent mechanism. Objective: To examine the effects of augmenting NUB1 in HD mouse striatum on mHTT levels. Methods: Striata of HDQ175/Q7 mice were injected at 3 months of age with recombinant AAV2/9 coding for NUB1 or GFP under the control of the neuron specific human synapsin 1 promoter and examined 6 months post-injection for levels of huntingtin, the striatal markers DARPP32 and PDE10A, the astrocyte marker GFAP, and the autophagy and mHTT aggregate marker P62 using immunolabeling of brain sections and Western blot assay of striatal subcellular fractions. Results: By Western blot human HD brain had only one of the two variants of NUB1 present in human control brain. In striatum of WT and HD mice NUB1 was localized in medium size neurons and enriched in the nucleus of large neurons. In the striatum of NUB1 injected HD mice, there was widespread neuronal distribution of exogenous NUB1 labeling and protein levels were ∼2.5-fold endogenous levels. DARPP32 and GFAP distribution and levels were unchanged but PDE10A levels were lower in crude homogenates and P62 was increased in nuclear enriched P1 fractions. Elevating NUB1 did not change levels of full-length mHTT or the number and size of mHTT (S830) positive nuclear inclusions. Conclusion: Findings suggest that increasing NUB1 protein in striatal neurons of HDQ175/Q7 mice in vivo may be relatively safe but is ineffective in reducing mHTT. Increased NUB1 expression in HD striatum alters PDE10A and P62 which are known to be influenced by mHTT.
