Affiliations: State Key Laboratory of Pollution Control and Resource
Reuse, School of the Environment, Nanjing University, Nanjing 210093,
China | Department of Ecology and Biodiversity, University of
Hong Kong, Hong Kong, China
Abstract: The purified terephthalic acid (PTA) petrochemical wastewater
molecular toxicity detected by use of Mouse Genome 430A 2.0 GeneChip was
conducted in this research. The toxic dose to male mice was 0.03
g/(kg·d) of PTA in the wastewater. The mice liver total RNA was isolated
as the temple for synthesis of cDNA and then the cDNA as the temple for
synthesis of cRNA. Hybridizing the cRNA with the target genes on the gene chip,
there were 232 genes expression levels up-regulated and 74 genes down-regulated
discovered obviously. The foremost 40 genes for both the highest and the lowest
expression levels involved endogenetic steroid and hormone metabolism, immune
system, the leukocyte activity and inflammation, detoxification in liver,
reproduction and growth hormone, regulation immune factors of anti-tumor and
anti-infection and cancer to the mice sampled. The data suggest the PTA
wastewater contained over 5 aromatics and their toxicities integrated were much
higher than the pure chemical PTA. And the pure chemical PTA toxicities data
cannot be used to evaluate the toxicity of the PTA wastewater instead.
Keywords: gene chip, PTA wastewater, male mice, molecular toxicity, predication of security
