Isolation, characterization and phylogenetic analysis of a bacterial strain capable of degrading acetamiprid

Article type: Research Article

Authors: YAO, Xiao-hua^; | MIN, Hang

Affiliations: College of Life Science, Zhejiang University, Hangzhou 310029, China | College of Agriculture, Guangxi University, Guangxi 530005, China

Note: [] Corresponding author. E-mail: [email protected]

Abstract: An aerobic bacterium, capable of degrading the new chloronicotine pesticide acetamiprid, was isolated from the sludge of pesticide factory after successive enrichment cultures and named strain FH2 which is a Gram-negative, rod-shaped, obligate aerobic organism with ((0.5–0.7) × (1.5–3.0))μm of cell size and with monotrichous flagellum. It was identified as a member of Pseudomonas sp. based on morphology, physio-biochemical properties, Biolog GN2, 16S rDNA sequence and phylogenetic characteristic analysis. The isolate could grow optimally at pH 7.0 and 30°C in acetamiprid-mineral medium with 800 mg/L concentration. About 53.3% acetamiprid was degraded by strain FH2 after incubation for 14 d in acetamiprid-mineral medium and nearly 96.7% degraded when incubated in acetamiprid-yeast mineral medium at 30°C for 14 d. This paper describes phylogenetic and degradation characterization of a pure bacterium being able to mineralize acetamiprid for the first time.

Keywords: acetamiprid, biodegradation, characterization, isolation, Pseudomonas sp., 16S rDNA

Journal: Journal of Environmental Sciences, vol. 18, no. 1, pp. 141-146, 2006