Affiliations: School of Environmental Science and Engineering,
Shanghai Jiao Tong University, Shanghai 200240, China | Institute of Applied Biochemistry, University of
Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-0006, Japan | National Institute for Environmental Studies, 16-2,
Onogawa, Tsukuba, Ibaraki 305-8506, Japan
Abstract: A molecular biology method, fluorescent in situ hybridization (FISH),
in which the pre-treatment was improved in allusion to the media of the
constructed wetlands (CW), e.g. the soil and the grit, was used to investigate
the vertical distribution characteristics of ammonia-oxidizing bacteria (AOB)
quantity and the relation with oxidation-reduction potential (ORP) in the Typha
latifolia constructed wetlands under three different loadings in summer from
May to September. Results showed that the quantity of the AOB decreased in the
Typha latifolia CW with the increase of vertical depth. However, the AOB
quantity was 2–4 times the quantity of the control in the root area.
Additionally, ORP in the rhizosphere was found to be higher than other areas,
which showed that Typha latifolia CW was in an aerobic state in summer when
using simulated non-point sewage at the rural area of Taihu Lake in China and
small town combined sewage.
