Effect of Cd on GSH and GSH-related enzymes of Chlamydomonas sp. ICE-L existing in Antarctic ice

Article type: Research Article

Authors: DING, Yu^; | MIAO, Jin-lai | LI, Guang-you | WANG, Quan-fu | KAN, Guang-feng | WANG, Guo-dong

Affiliations: Marine Life College, Ocean University of China, Qingdao 266003, China | Key Lab. of Marine Bioactive Substances, SOA, Qingdao 266061, China | Fisheries College, Zhanjiang Ocean University, Zhanjiang 524025, China

Note: [] Corresponding author. E-mail: [email protected]

Abstract: Glutathione (GSH) and GSH-related enzymes play a great role in protecting organisms from oxidative damage. The GSH level and GSH-related enzymes activities were investigated as well as the growth yield and malonyldialdehyde (MDA) content in the Antarctic ice microalga Chlamydomonas sp. ICE-L exposure to the different cadmium concentration in this paper. The results showed that the higher concentration Cd inhibited the growth of ICE-L significantly and Cd would induce formation of MDA. At the same time, it is clear that GSH level, glutathione peroxidases (GPx) activity and glutathione S-transferases (GST), activity were higher in ICE-L exposed to Cd than the control. But GR activity dropped notably when ICE-L were cultured in the medium containing Cd. Increase of GSH level, GPx and GST activities acclimate to oxidative stress induced by Cd and protect Antarctic ice microalga Chlamydomonas sp. ICE-L from toxicity caused by Cd exposure. These parameters may be used to assess the biological impact of Cd in the Antarctic pole region environment.

Keywords: antarctic ice microalgae, Chlamydomonas sp. ICE-L, glutathione, glutathione peroxidase, glutathione reductase, glutathione S-transferase, cadmium

Journal: Journal of Environmental Sciences, vol. 17, no. 4, pp. 667-671, 2005