Affiliations: College of Life Sciences, Henan Normal University,
Xinxiang 453002, China | Research Center for EcoEnvironmental Sciences, Chinese
Academy of Sciences, Beijing 100085, China | Henan Key Laboratory for Environmental Pollution
Control, Henan Normal University, Xinxiang 453002, China
Abstract: Since the ability to degrade lignin with one kind of white-rot fungi
or bacteria was very limited, superior mixed flora's ability to degrade lignin
was investigated by an orthogonal experiment in this paper. The results showed
that superior mixed flora reinforced the ability to degrade lignin, the
degradation rates of both sample 9 and 10 were beyond 80% on the day 9. The
cooperation between lignin peroxidase (LiP), Mn-dependent peroxidase (MnP) and
laccase (Lac) for lignin degradation was also studied. By examining the
activities of three enzymes produced by superior mixed flora, it was found that
Lac was a key enzyme in the process of biological degradation of lignin but Lip
was not; the enzyme activity ratios of Lac/MnP and Lac/LiP were significantly
correlative with the degradation rate of lignin at the 0.01 level; and the
ratio of MnP/LiP was an important factor affecting the degradation rate of
lignin.
