Affiliations: Institute of Applied Ecology, Chinese Academy of
Sciences, Shenyang 110016, China | Graduate School of Chinese Academy of Sciences,
Beijing 100010, China | College of Land and Environment, Shenyang Agricultural
University, Shenyang 110161, China
Abstract: The phytotoxicity of chlorobenzoic acids (CBAs) was studied and the
biochemical endpoints' suitability and sensibility was evaluated. Two
terrestrial plant species in the same family were exposed to different
concentrations of CBAS and tested their germination according to the guideline
of Organization for Economic Cooperation and Development (OECP, 1984). The
results showed that CBA dose-inhibition rate of classical endpoint had the
distinct linear relationship in the range of 10%–50% inhibition rate for
root elongation (p < 0.01), and the dose variances of CBAs had the greater
influence on the inhibition rate of germination than on inhibition rate of root
elongation. The CBA dose half effect concentration-inhibition rate of two
antioxidant enzyme activity superoxide dismutase (SOD) and catalase (CAT) had
the quadratic relationship, and CBA dose-inhibition rate of the peroxides (POD)
activity had the linear relationship (p <0.05). Comparing the half effect
concentration (EC_{50}) of two kinds of endpoints, the POD
activity was more sensitive than classical endpoint, however, SOD and CAT
activity were not sensitive in the experiment.
