Abstract: As an oil-decomposable mixture of two bacteria strains (Bacillus
sp. and Pseudomonas sp.), Y3 was isolated after 50 d domestication under the
condition that oil was used as the limited carbon source. The decomposing rate
by Y3 was higher than that by each separate individual strain, indicating a
synergistic effect of the two bacteria. Under the conditions that T =
25–40°C, pH = 6–8, HRT (Hydraulic retention time) = 36 h and the oil
concentration at 0.1%, Y3 yielded the highest decomposing rate of 95.7%. Y3
was also applied in an organic waste treatment machine and a certain rate of
activated bacteria was put into the stuffing. A series of tests including
humidity, pH, temperature, C/N rate and oil percentage of the stuffing were
carried out to check the efficacy of oil-decomposition. Results showed that the
oil content of the stuffing with inoculums was only half of that of the
control. Furthermore, the bacteria were also beneficial to maintain the
stability of the machine operating. Therefore, the bacteria mixture as well as
the machines in this study could be very useful for waste treatment.
