Affiliations: Institute of Applied Ecology, Chinese Academy of
Sciences, Shenyang 110016, China | School of the Chinese Academy of Sciences, Beijing
100039, China
Abstract: To exploit peashrub resources in Ordos as fodders, it is very
crucial to realize industrial production of cheap cellulase of high activity by
optimizing culture technology, especially culture substrate. In this study, a
new prescription experiment based on uniform design ideal was invented and
successfully applied in the solid fermentation of Trichoderma koningii F244,
which being performed with two different temperature degrees. The activities of
FPA, cotton lyase, CMCase and β-glucosidase were assayed and then
mathematical models of enzymatic activities, which were figured out by
Unconstraint Mathematical Programming, were developed by Multivariate
Regression Program of SPSS10.0. Enzymatic activities of optimized substrate
prescriptions corresponding to mathematical models were forecasted to determine
an ideal substrate prescription. It is revealed that in solid fermentation,
Tween80 has negative effect on cellulase production. Furthermore, the ideal
prescription for cellulase complex production by Trichoderma koningii F244 was
straw powder 16.9%,wheat bran 26.5%,
(NH_4)_2SO_4 9.5% and water 47.1%, whose
corresponding cellulase activity was expected to be at the same high level with
that of Trichoderma reesei Q9414 on its own recommended substrate. Especially,
goats mainly fed on peashrub tissues mixed with cellulase complex of this
prescription and culture technology, got an incremental ratio of 0.3 kg/d,
which brought a very promising feeding prospect for local peashrub resource. By
populization of this cellulase complex, it can integrate living standard,
economic construction of local residents into vegetational restoration tightly
and thus this paper will be very meaningful to be use for reference for western
China like Ordos to realize its sustainable development of economy, society and
environment.
