Affiliations: State Key Laboratory of Environmental Aquatic
Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of
Sciences, Beijing 100085, China | Qingdao University of Science & Technology,
Qingdao 266042, China
Abstract: A simple and Iow-cost method to obtain cyanobacterial toxin
microcystin-LR (MC-LR) was developed. A new strain of Microcystis aeruginosa,
named DC-1, producing microcystin-LR but not microcystin-RR, was separated from
the field blooming algae samples of Dianchi Lake, in southwest of China.
Following three times' freeze and thaw treatment, the cultivated DC-1 cells were
extracted with 40% methanol in water. The extract was centrifuged and the
supernatant applied to a Hydrophilic-Lipophilic Balance (HLB) SPE cartridge.
Eluted impurities with a certain gradient from 30% to 50% methanol in water,
MC-LR was finally eluted from the HLB cartridge with 60% methanol in water,
and samples containing 3.85% to 14.8% of MC-LR were obtained. These MC-LR
samples may be used in adsorption and biodegradation experiments instead of
using expensive standard reagents.
