Abstract: The main effects of pollutions including acid rain,
Cu^{2+}, atrazine and their combined products on the
activities of urease, invertin, acid phosphatase and catalase were studied by
means of orthogonal test. The results showed that H^^+ and
Cu^{2+} had significant influence on the activities of four
enzymes and the ability of their inhibiting followed the order:
H^+ � Cu^{2+}.
Al^{3+} and atrazine only had litter effects on the activity
of urease and phosphatase, respectively. Furthermore, interaction analysis
revealed that Cu^{2+}-H^+ affected on the
activity of acid phosphatase significantly and antagonism on invertin and
urease, Cu^{2+}-atrazine only exhibited the synergism on the
activity of acid phosphatase. But atrazine-H^+ had
non-interaction within the investigated concentration range. Among four
enzymes, acid phosphatase was the most sensitive one to the contaminations.