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Article type: Research Article
Authors: Apostolopoulos, G.a; * | Tsinopoulos, S.b | Dermatas, E.a
Affiliations: [a] Department of Electrical Engineering and Computer Technology, University of Patras, Kato Kastritsi, 26500 Patras, Greece | [b] Mechanical Department, TEI of Patras, Patras, Greece
Correspondence: [*] Corresponding author. Tel.: +30 2610 991722; Fax: +30 2610 991855; E-mail: [email protected].
Abstract: In this paper, a novel method for the estimation of the human Red Blood Cell (RBC) size using light scattering images is presented. The information retrieval process includes, image normalization, a two-dimensional Discrete Cosine Transformation (DCT2) or Wavelet transformation (DWT2), and a Radial Basis Neural Network (RBF-NN) estimates the RBC geometrical properties. The proposed method is evaluated in both regression and identification tasks when three important geometrical properties of the human RBC are estimated using a database of 1575 simulated images generated with the boundary element method. The experimental setup consists of a light beam at 632.8 nm and moving RBCs in a thin glass and additive noise distortion is simulated using white Gaussian noise from 60 to 0 dB SNR. The regression and identification accuracy of actual RBC sizes is estimated using three feature sets, giving a mean error rate less than 1 percent of the actual RBC size, in case of noisy image data at 10 dB SNR or better, and more than 97 percent mean identification rate.
Keywords: Human red blood cell, neural network, light scattering, DCT2, DWT2, radial basis function, boundary element method
DOI: 10.3233/JCM-2009-0254
Journal: Journal of Computational Methods in Sciences and Engineering, vol. 9, no. 1-2, pp. 19-30, 2009
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