Factors affecting in vitro and in vivo antioxidant effects: Experimental conditions and nature of oxidants determine antioxidant efficacy

Issue title: The Application of Modern Chromatographic and Spectroscopic Techniques for the Analysis of Berry Fruits

Guest editors: Prof. Etsuo Niki, Prof. Esra Capanoglu and Dr. Elwira Sieniawska

Article type: Review Article

Authors: Niki, Etsuo^{; *}

Affiliations: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo, Japan

Correspondence: [*] Corresponding author: Etsuo Niki, 7-17-7 Kitakarasuyama, Setagaya, Tokyo 157 0061, Japan. Tel./Fax: +81 353132555; E-mails: [email protected] and [email protected].

Abstract: Reactive oxygen and nitrogen species have been implicated in the onset and progression of various diseases and the role of antioxidants in the maintenance of health and prevention of diseases has received much attention. The action and effect of antioxidants have been studied extensively under different reaction conditions in multiple media. The antioxidant effects are determined by many factors. This review aims to discuss several important issues that should be considered for determination of experimental conditions and interpretation of experimental results in order to understand the beneficial effects and limit of antioxidants against detrimental oxidation of biological molecules. Emphasis was laid on cell culture experiments and effects of diversity of multiple oxidants on antioxidant efficacy.

Keywords: Antioxidant, cell culture medium, oxygen concentration, reactive oxygen species

DOI: 10.3233/JBR-200695

Journal: Journal of Berry Research, vol. 11, no. 4, pp. 601-609, 2021