Effects of Hepatorenal Function on Urinary Alzheimer-Associated Neuronal Thread Protein: A Laboratory-Based Cross-Sectional Study Among the Older Chinese Population

Article type: Research Article

Authors: Jin, He^{a; b} | Yang, Qiu^b | Chen, Guodong^b | Zhang, Wei^a | Wu, Yanchuan^a | Wang, Rong^{a; c; d; *}

Affiliations: [a] Central Laboratory, Xuanwu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Beijing, China | [b] Clinical Laboratory, Affiliated Hospital of Guilin Medical University, Guilin, China | [c] Center of Alzheimer’s Disease, Beijing Institute for Brain Disorders, Beijing, China | [d] National Clinical Research Center for Geriatric Disorders, Beijing, China

Correspondence: [*] Correspondence to: Rong Wang, PhD; No. 45, Changchun Street, Beijing 100053, P.R. China. Tel./Fax: +86 1063159572; E-mail: [email protected].

Abstract: Background:Urinary Alzheimer-associated neuronal thread protein (AD7c-NTP) is a biomarker for the early diagnosis of Alzheimer’s disease (AD). It remains unclear whether hepatorenal function affects the urinary AD7c-NTP level. Objective:To evaluate the effects of hepatorenal function on urinary AD7c-NTP level. Methods:We enrolled 453 participants aged 60–100 years. An automated chemistry analyzer was used to determine the indicators of serum hepatorenal function. Enzyme-linked immunosorbent assay was used to measure the urinary AD7c-NTP level. Results:Spearman’s correlation analysis showed a negative correlation between urinary AD7c-NTP levels and indicators of hepatorenal function, including albumin (r = –0.181, p < 0.001), albumin/globulin ratio (r = –0.224, p < 0.001), cholinesterase (r = –0.094, p = 0.046), total carbon dioxide (r = –0.102, p = 0.030), and glomerular filtration rate (r = –0.260, p < 0.001), as well as a positive correlation with globulin (r = 0.141, p = 0.003), aspartate transaminase (r = 0.186, p < 0.001), blood urine nitrogen (r = 0.210, p < 0.001), creatinine (r = 0.202, p < 0.001), uric acid (r = 0.229, p < 0.001), and cystatin C (r = 0.265, p < 0.001). The least absolute shrinkage and selection operator (LASSO) regression analysis and multiple linear regression model analyses showed that the statistically significant hepatorenal indicators for predicting AD7c-NTP were A/G (p = 0.007), AST (p = 0.002), BUN (p = 0.019), and UA (p = 0.003). Conclusions:The effects of hepatorenal indicators should be considered when using urinary AD7c-NTP levels in clinical settings.

Keywords: Alzheimer’s disease, Alzheimer-associated neuronal thread protein, kidney, liver, hepatorenal indicator, urine

DOI: 10.3233/JAD-240148

Journal: Journal of Alzheimer's Disease, vol. Pre-press, no. Pre-press, pp. 1-11, 2024