Affiliations: [a]
INRS-Centre Armand-Frappier Santé-Biotechnologie, Laval, QC, Canada
| [b]
Institute of Nutrition and Functional Foods, Québec, QC, Canada
| [c]
Higher Institute of Biotechnology of Beja, University of Jendouba, Beja, Tunisia
| [d]
Department of Medicine, Geriatric Division, Research Center on Aging, Sherbrooke University, Sherbrooke, QC, Canada
| [e]
Centre d’Excellence Sur le Vieillissement de Québec, CHU de Québec-Université Laval Research Centre, VI-TAM-Centre de Recherche en Santé Durable, Québec, QC, Canada
| [f] Faculty of Pharmacy, Laval University, Québec, QC, Canada
Correspondence:
[*]
Correspondence to: Charles Ramassamy, Institut National de Recherche Scientifique–Centre Armand-Frappier Santé-Biotechnologie, Laval, QC H7V 1B7, Canada. Tel.: +1 450 687 5010; E-mail: [email protected].
Note: [1] These authors contributed equally to this work.
Abstract: Background:The perplex interrelation between circulating extracellular vesicles (cEVs) and amyloid-β (Aβ) deposits in the context of Alzheimer’s disease (AD) is poorly understood. Objective:This study aims to 1) analyze the possible cross-linkage of the neurotoxic amyloid-β oligomers (oAβ) to the human cEVs, 2) identify cEVs corona proteins associated with oAβ binding, and 3) analyze the distribution and expression of targeted cEVs proteins in preclinical participants converted to AD 5 years later (Pre-AD). Methods:cEVs were isolated from 15 Pre-AD participants and 15 healthy controls selected from the Canadian Study of Health and Aging. Biochemical, clinical, lipid, and inflammatory profiles were measured. oAβ and cEVs interaction was determined by nanoparticle tracking analysis and proteinase K digestion. cEVs bound proteins were determined by ELISA. Results:oAβ were trapped by cEVs and were topologically bound to their external surface. We identified surface-exposed proteins functionally able to conjugate oAβ including apolipoprotein J (apoJ), apoE and RAGE, with apoJ being 30- to 130-fold higher than RAGE and apoE, respectively. The expression of cEVs apoJ was significantly lower in Pre-AD up to 5 years before AD onset. Conclusion:Our findings suggest that cEVs might participate in oAβ clearance and that early dysregulation of cEVs could increase the risk of conversion to AD.
