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Article type: Short Communication
Authors: Mabrouk, Ranaa; 1; * | Gotkiewicz, Mariaa; 1 | Rauramaa, Tuomasb; c | Tanila, Heikkia
Affiliations: [a] A.I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland | [b] Department of Pathology, Kuopio University Hospital, Kuopio, Finland | [c] Unit of Pathology, Institute of Clinical Medicine, University of Eastern Finland, Kuopio, Finland
Correspondence: [*] Correspondence to: Rana Mabrouk, A.I. Virtanen Institute, University of Eastern Finland, 70210 Kuopio, Finland. E-mail: [email protected].
Note: [1] These authors contributed equally to this work.
Abstract: DAPI is conventionally used as a nuclear stain for cells in culture or tissue. Here we demonstrate that it binds specifically to the β-sheet core of amyloid-β plaques but not diffuse amyloid-β at the plaque periphery. The specific DAPI induced blue fluorescence is much stronger than amyloid plaque autofluorescence. DAPI staining of fibrillar amyloid deposit may yield a misleading impression of damaged or dying cells. On the other hand, it provides a handy and low-cost means of staining compact amyloid plaques together with cell nuclei in double or triple immunofluorescent studies.
Keywords: Alzheimer’s disease, amyloid-β plaques, autofluorescence, DAPI
DOI: 10.3233/JAD-220072
Journal: Journal of Alzheimer's Disease, vol. 88, no. 3, pp. 949-955, 2022
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