High Dietary Advanced Glycation End Products Impair Mitochondrial and Cognitive Function

Article type: Research Article

Authors: Akhter, Firoz^{a; c} | Chen, Doris^a | Akhter, Asma^{a; c} | Sosunov, Alexander A.^b | Chen, Allen^a | McKhann, Guy M.^b | Yan, Shi Fang^{a; b; *} | Yan, Shirley ShiDu^{a; c; *}

Affiliations: [a] Department of Pharmacology and Toxicology and Higuchi Biosciences Center, University of Kansas, Lawrence, KS, USA | [b] Department of Neurological Surgery and Surgery, Columbia University, New York, NY, USA | [c] Department of Surgery, Columbia University, New York, NY, USA

Correspondence: [*] Correspondence to: Shirley ShiDu Yan, MD, or Shi Fang Yan, MD, Department of Surgery, Columbia University, New York, NY 10032, USA. Tel.: +1 212 342 0494; E-mails: [email protected] or [email protected]

Abstract: Background:Advanced glycation end products (AGEs) are an important risk factor for the development of cognitive decline in aging and late-onset neurodegenerative diseases including Alzheimer’s disease. However, whether and how dietary AGEs exacerbate cognitive impairment and brain mitochondrial dysfunction in the aging process remains largely unknown. Objective:We investigated the direct effects of dietary AGEs on AGE adducts accumulation, mitochondrial function, and cognitive performance in mice. Methods:Mice were fed the AGE+ diet or AGE– diet. We examined levels of AGE adducts in serum and cerebral cortexes by immunodetection and immunohistochemistry, determined levels of reactive oxygen species by biochemical analysis, detected enzyme activity associated with mitochondrial respiratory chain complexes I & IV and ATP levels, and assessed learning and memory ability by Morris Water Maze and nesting behavior. Results:Levels of AGE adducts (MG-H1 and CEL) were robustly increased in the serum and brain of AGE+ diet fed mice compared to the AGE– group. Furthermore, greatly elevated levels of reactive oxygen species, decreased activities of mitochondrial respiratory chain complexes I & IV, reduced ATP levels, and impaired learning and memory were evident in AGE+ diet fed mice compared to the AGE– group. Conclusion:These results indicate that dietary AGEs are important sources of AGE accumulation in vivo, resulting in mitochondrial dysfunction, impairment of energy metabolism, and subsequent cognitive impairment. Thus, reducing AGEs intake to lower accumulation of AGEs could hold therapeutic potential for the prevention and treatment of AGEs-induced mitochondrial dysfunction linked to cognitive decline.

Keywords: Advanced glycation end products, methylglyoxal, mitochondrial and cognitive dysfunction

DOI: 10.3233/JAD-191236

Journal: Journal of Alzheimer's Disease, vol. 76, no. 1, pp. 165-178, 2020