Affiliations: Department of Pharmacognosy, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China
Correspondence:
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Correspondence to: Ping Li, PhD, Professor, Director, Department of Pharmacognosy, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjia Lane, Nanjing 210009, China. Tel./Fax: +86 25 83271379; E-mail: [email protected] and Xiaojun Xu, PhD, Associate Professor, Department of Pharmacognosy, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjia Lane, Nanjing 210009, China. Tel.: +86 25 83271382; E-mail: [email protected].
Abstract: Triptolide, a biologically active natural product from Tripterygium wilfordii, protects neurons from inflammation-mediated damage. Our results showed for the first time that triptolide inhibited the expression of CXCR2 and presenilin in a neuroblastoma cell line SHSY5Ysw. Moreover, triptolide potently inhibited amyloid-β1-42 production with IC50 value of 30 pM in HEK293sw cells or 2 nM in SHSY5Ysw cells, respectively. We also demonstrated that triptolide prevented primary cortical neurons from chemokine CXCL1-induced cytotoxicity. Therefore, our study indicates that the neural protective effect of triptolide is largely mediated by inhibiting CXCR2 activity.
