Blocking the Apolipoprotein E/Amyloid-β Interaction Reduces Fibrillar Vascular Amyloid Deposition and Cerebral Microhemorrhages in TgSwDI Mice

Article type: Research Article

Authors: Yang, Jing^a | Ji, Yong^a | Mehta, Pankaj^b | Bates, Kristyn A.^{a; e} | Sun, Yanjie^a | Wisniewski, Thomas^{a; c; d; *}

Affiliations: [a] Department of Neurology, New York University School of Medicine, New York, NY, USA | [b] New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York, NY, USA | [c] Department of Pathology, New York University School of Medicine, New York, NY, USA | [d] Department of Psychiatry, New York University School of Medicine, New York, NY, USA | [e] School of Exercise, Biomedical and Health Science, Edith Cowan University, Western Australia

Correspondence: [*] Correspondence to: Thomas Wisniewski, M.D., New York University School of Medicine, Millhauser Laboratory, Room HN419, 550 First Avenue, New York, NY 10016, USA. Tel.: +1 212 263 7993; Fax: +1 212 263 7528. E-mail: [email protected].

Abstract: The accumulation of amyloid-β (Aβ) peptides as toxic oligomers, amyloid plaques, and cerebral amyloid angiopathy (CAA) is critical in the pathogenesis of Alzheimer's disease (AD). The binding of Aβ peptides to apolipoprotein E (ApoE) plays an important role in modulation of amyloid deposition and clearance. We have shown that blocking the Aβ/ApoE interaction with Aβ12-28P, a nontoxic blood-brain-barrier permeable and non-fibrillogenic synthetic peptide, constitutes a novel therapeutic approach for AD by reducing Aβ parenchymal deposition. In the present study, we investigate this therapeutic effect on CAA in the transgenic (Tg) AD mice model (TgSwDI), which expresses Swedish (K670N/M671L), Dutch (E693Q)/Iowa (D694N) AβPP mutations. These mice develop abundant CAA beginning at the age of 6 months. Behavioral results show that A12-28P treated TgSwDI AD mice performed the same as wild-type mice, whereas vehicle treated TgSwDI were impaired in spatial memory. Furthermore, this treatment resulted in a significant reduction of total amyloid burden, especially the fibrillar vascular amyloid burden, which importantly was accompanied by a reduction in microhemorrhages and neuroinflammation. Measurement of Aβ levels in the brain homogenate revealed a significant decrease in both the total amount of Aβ and Aβ oligomer levels in A12-28P treated TgSwDI mice. These findings suggest that blocking the Aβ/ApoE interaction is a highly effective therapeutic approach for vascular amyloid deposition, in contrast to some other therapeutic approaches.

Keywords: Alzheimer's disease, amyloid-β, apolipoprotein E, cerebral amyloid angiopathy, microhemorrhages, microglia, neuroinflammation

DOI: 10.3233/JAD-2011-101401

Journal: Journal of Alzheimer's Disease, vol. 24, no. 2, pp. 269-285, 2011