Abstract: Fibrillar amyloid-β protein (fAβ) is the principal component of amyloid plaques in the brains of patients with Alzheimer's disease (AD). We have recently reported that activity of trypsin is inhibited by fAβ and that trypsin can bind to fAβ. Neprilysin and insulysin are important proteases for the clearance of soluble Aβ. Here, we report that fAβ also binds to neprilysin and insulysin, which results in the inhibition of their proteolytic activities. These findings suggest that clearance of soluble Aβ may be defective in AD because of binding of proteases to amyloid plaques, leading to inactivation of proteases that are required for catabolism of Aβ. The identification of compounds that can inhibit binding of proteases to fAβ may, therefore, be of significance for therapeutic intervention in AD. Congo red and Thioflavin T are widely used for histopathological examination of amyloid plaques because of their strong affinity to fibrillar amyloid proteins. We examined the effect of Congo red and Thioflavin T (potent fAβ-binding compounds) on the binding of different proteases to fAβ. While Congo red inhibited the binding of trypsin, neprilysin and insulysin to fAβ, Thioflavin T did not have any effect. The effect of Congo red was concentration-dependent and the inhibitory effect was in the order of trypsin > insulysin > neprilysin. When the effect of prebound-Congo red to fAβ was examined, trypsin was unable to bind to this complex suggesting that Congo red may have better affinity than trypsin for binding to fAβ. Based on these results, we propose that the inhibition of binding of proteases to amyloid plaques may help in reducing the deposition of Aβ in AD.
