Affiliations: [a] Research Center, Methodist University Center IPA, Porto Alegre, Brazil | [b] Department of Health Basic Science, Federal University of Health Sciences of Porto Alegre, Porto Alegre, Brazil | [c] Physical Education School, Federal University of Rio Grande do Sul, Porto Alegre, Brazil | [d] Laboratory of Immunogenetics, Federal University of Rio Grande do Sul, Porto Alegre, Brazil | [e] Federal Institution of Education, Science and Technology of RS, Porto Alegre, Brazil
Correspondence:
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Corresponding author: Alessandra Peres, Cellular and Molecular Immunology Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite Street, 245, Lab 206. 90050-170, Porto Alegre, RS, Brazil. Tel.: +55 51 3303 8746; E-mail:[email protected]
Abstract: OBJECTIVE: To compare the expression of C-C Chemokine receptor type
5 (CCr5) on monocytes and T cells in soccer players who were submitted to
isokinetic concentric or eccentric exercise. METHODS: Fourteen male athletes from the U-20 soccer category of a
first division Brazilian soccer team (ages 17.75 ± 1.21 years) were
randomized into concentric exercise (CON, n = 7) or eccentric (ECC, n = 7)
isokinetic exercise groups. The peripheral frequency of CD3+ CCR5 and
CD14+ CCR5+ was evaluated before, immediately after, and 24 h after each
exercise bout using flow cytometry. RESULTS: Progressive decreases were found in total CCr5 in ECC
groups, with significant differences between baseline and 24 h after
(p = 0.023). Both ECC and CON groups induced a significant decrease in CCr5
expression on the cell surface of CD14+ monocytes 24 h after bout
(p < 0.05), with the ECC exercise associated with a more pronounced decrease
(p < 0.05). In CD3+ T cells, CCr5 expression increased immediately after
ECC but not in the CON exercise, returning to baseline values 24 h after
(p < 0.05). CONCLUSIONS: CCr5 is highly sensitive to physical exercise and the
modulation of its expression on cell surface may be related to migration of
the cells to damaged tissue.
