Affiliations: Institute of Molecular Biomedicine, Comenius
University, Bratislava, Slovakia | Department of Molecular Biology, Comenius University,
Bratislava, Slovakia | Institute of Pathological Physiology, Comenius
University, Bratislava, Slovakia
Note: [] Corresponding author: Peter Celec, Institute of Molecular
Biomedicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia.
Tel.: +421 2 59357371; Fax: +421 2 59357631; E-mail: [email protected]
Abstract: BACKGROUND: Salivary markers of oxidative stress and antioxidant
status represent promising tool for the research of oral diseases. One of the
criteria is the validation of these biomarkers from the perspective of the
confounding and modifying factors. AIM: To examine the effect of circadian rhythm, tooth-brushing and
ascorbic acid treatment on selected salivary markers of oxidative and carbonyl
stress, and antioxidant status. SUBJECTS AND METHODS: Whole unstimulated saliva samples were
collected from 19 healthy participants three times during a day, before and
after tooth-brushing, and before and after the administration of vitamin C (250
mg). Advanced oxidation protein products (AOPP), thiobarbituric acid reactive
substances (TBARS), advanced glycation end products (AGEs), ferric reducing
antioxidant power (FRAP) and total antioxidant capacity (TAC) were measured.
RESULTS: Salivary AGEs levels varied significantly during the day
(p< 0.05) with the highest concentrations in the morning. FRAP levels varied
during the day (p < 0.01) with the highest concentrations in the afternoon.
Tooth-brushing decreased AGEs (p< 0.05) and TBARS levels (p< 0.01) and
increased FRAP levels (p< 0.05). Single intake of vitamin C significantly
decreased AGEs (p < 0.001) and increased both FRAP (p< 0.01) and TAC
(p< 0.01) concentrations. CONCLUSION: Significant daily variations were observed in salivary
AGEs and FRAP levels. Tooth-brushing and treatment with vitamin C decreased
carbonyl stress and increased the antioxidant status. These results are
important from the perspective of using saliva for the research of oral
diseases.
