Utility of OCT3/4, TSPY and β-catenin as biological markers
for gonadoblastoma formation and malignant germ cell tumor development in
dysgenetic gonads
Affiliations: Molecular and Cellular Morphology Laboratory, Escuela
Superior de Medicina, Instituto Politécnico Nacional, Mexico City,
Mexico | Morphology Department, Facultad de Medicina
Veterinaria y Zootecnia, UNAM, Mexico City, Mexico | Human Genetics Department, Hospital General de
México, Mexico City, Mexico | Pathology Department, Hospital Infantil de
México-Federico Gémez, Mexico City, Mexico | Biology Development Department, Hospital Infantil de
México-Federico Gómez, Mexico City, Mexico | Urology Department, Hospital Infantil de
México- Federico Gómez, Mexico City, Mexico | Endocrinology Department, Hospital Infantil de
México-Federico Gómez, Mexico City, Mexico | Facultad de Medicina Universidad Nacional
Autónoma de México, Mexico City, Mexico | Pathology Department, Hospital General de
México, Mexico City, Mexico
Note: [] Corresponding author: Gloria Queipo, Human Genetics Department,
Hospital General de Mexico, Mexico City, Mexico; Facultad de Medicina
Universidad Nacional Autonoma de Mexico, Mexico City, Mexico. Dr. Balmis 142
Col, Doctores CP 06766 Mexico DF. Tel.: +52 5527892000 (1278/1279); E-mail:
[email protected], [email protected]
Abstract: BACKGROUND: Gonadoblastoma (GB) is regarded as an in situ form of
germ cell tumor in dysgenetic gonads, and 30% of patients with GB develop a
dysgerminoma/seminoma tumor. OBJECTIVE: Determine whether OCT3/4 and β-catenin are expressed
in dysgenetic gonads before GB development and whether TSPY participates in the
OCT3/4-β-catenin pathways in the malignant invasive behavior. METHODS: dysgenetic gonads of Disorders of sex differentiation (DSD)
patients with mixed gonadal dysgenesis were analyzed by immunohistochemistry
and immunofluorescence for comparison with GB and dysgerminoma/seminoma. RESULTS: Our results suggest that the development of GB is secondary
to the interaction of OCT3/4 and TSPY, that β-catenin does not participate
in this process. CONCLUSIONS: The use of this biological markers detects the
potential high risk gonads.
