Affiliations: Environmental Biochemistry and Molecular Biology
Laboratory, Department of Biochemistry, University College of Medical Sciences
and GTB Hospital, University of Delhi, Dilshad Garden, Delhi, India | Department of Surgery, University College of Medical
Sciences and GTB Hospital, University of Delhi, Dilshad Garden, Delhi,
India | Division of Biochemistry and Biotechnology, National
Centre for Communicable Diseases, 22, Sham Nath Marg, Delhi, India | Director and CEO, Delhi State Cancer Institute,
Dilshad Garden, Delhi, India
Note: [] Corresponding author: Dr. B. D. Banerjee, M.Phil, PhD,
Professor and Head (Medical Biochemistry, Faculty of Medical Sciences, DU).
Department of Biochemistry, University College of Medical Sciences and Guru
Tegh Bahadur Hospital, University of Delhi, Dilshad Garden, Delhi-110 095,
India. Tel.: +91 11 22135362; Fax: +91 11 22590495; E-mail: [email protected]
Abstract: Association of glutathione S-transferase (GST) M1
and T1 deletions with benign prostate hyperplasia (BPH) and
prostate cancer is well reported. These enzymes metabolize numerous toxins thus
protecting from oxidative injury. Oxidative stress has been associated with
development of BPH and prostate cancer. The present study was designed to
analyze role of GST deletions in development of oxidative stress in these
subjects. GSTs are responsible for metabolism of toxins present in tobacco
therefore effect of tobacco usage in study groups was also studied. Three
groups of subjects: BPH (57 patients), prostate cancer (53 patients) and
controls (46 subjects) were recruited. Genotyping was done using a multiplex
polymerase chain reaction (PCR) method. Malondialdehyde (MDA) levels as marker
of oxidative stress were estimated by measuring thiobarbituric acid reactive
substance (TBARS) in plasma. Based on genotyping, subjects were categorized
into: GSTM1+/GSTT1+, GSTM1-/GSTT1+, GSTM1+/GSTT1- and
GSTM1-/GSTT1-. Significantly higher plasma MDA levels were noticed in
GSTM1-/GSTT1- as compared to GSTM1+/GSTT1+ in all study groups. Double
deletion (GSTM1-/GSTT1-) is associated with higher oxidative stress which
might play a role in the pathogenesis of BPH and prostate cancer. However,
other markers of oxidative stress should be analyzed before any firm conclusion.
