Affiliations: Unidad Académica de Ciencias Químico
Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero,
México | Instituto de Investigación en Reumatología y
del Sistema Músculo Esquelético, Centro Universitario de Ciencias de la
Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México
Note: [] Corresponding author: Isela Parra-Rojas, PhD, Unidad
Académica de Ciencias Químico Biológicas, Universidad
Autónoma de Guerrero, Avenida Lázaro Cárdenas S/N, Ciudad
Universitaria, Chilpancingo, Guerrero, 39090, México. Tel./Fax: +52 747
472 5503; E-mail: [email protected]
Abstract: Interleukin-6 (IL-6) is a cytokine involved in inflammatory process,
as well as in glucose and lipid metabolism. Several studies of the biological
relevance of IL-6 gene polymorphisms have indicated a relationship with
cardiovascular disease. The aim of this study was to assess whether the –174
G/C and –572 G/C of IL-6 gene polymorphisms are associated with cardiovascular
risk factors in Mexican families. Ninety members of 30 Mexican families, in
which an index case (proband) had obesity, were included in the study. We
evaluated the body composition by bioelectrical impedance. Peripheral blood
samples were collected to determine biochemical and hematological parameters.
High sensitivity C- reactive protein levels were measurement for nephelometric
analysis. Screening for both polymorphisms studied was performed by PCR-RFLP.
In the parents, both polymorphisms were in Hardy-Weinberg's equilibrium. The
genotypes –174 GC/CC were associated with T2D (OR=1.23,
IC_{95%} levels of hsCRP (p=0.02), whereas
genotype –572 GG was associated with T2D (OR=1.24,
IC_{95%} 1.04–1.47) with an inflammatory state determined by the
increase in the leukocyte count (OR=1.24, IC_{95%}
1.02–1.51). The genotypes –174 GC/CC and –572 GG may confer susceptibility
for the development of subclinical inflammation and type 2 diabetes in Mexican families.
Keywords: Polymorphisms, Interleukin-6, diabetes, inflammation, family study
