Affiliations: Instituto de Investigación en
Reumatología y del Sistema Músculo Esquelético,
Departamento de Biología Molecular y Genómica, Centro
Universitario de Ciencias de la Salud, Universidad de Guadalajara | Instituto de Investigación en Genética
Molecular, Centro Universitario de la Ciénega, Universidad de
Guadalajara, Ocotlán, Jalisco, México
Note: [] Corresponding author: José Francisco Muñoz-Valle,
PhD., Insurgentes 244-1, Colonia Lomas de Atemajac, Zapopan, Jalisco, C.P.
45178, México. Tel.: +52 33 10585309; E-mail:
[email protected]
Abstract: To investigate the association of sICAM-1 and sVCAM-1 with ICAM1
721G>A and VCAM1 1238G>C polymorphisms and rheumatoid arthritis (RA)
clinical activity, sixty RA patients and 60 healthy non-related subjects (HS)
matched for age and sex were recruited. Soluble adhesion molecules were
determined by ELISA technique. Rheumatoid factor (RF), C reactive protein (CRP)
and the erythrocyte sedimentation rate (ESR) were measured by routine methods.
Disability and clinical activity was measured with Spanish-HAQ-DI and DAS28
scores, respectively. The ICAM1 and VCAM1 polymorphism were identified using
the PCR-RFLP procedure. Inter-group comparison showed increased levels of
sICAM-1 and sVCAM-1 in RA patients (284 and 481 ng/mL) versus HS (132 and 280
ng/mL); in the RA group, significant correlations between sVCAM-1 and RF (r =
0.402), ESR (r = 0.426), Spanish-HAQ-DI (r = 0.276), and DAS28 (r = 0.342) were
found, whereas sICAM-1 only correlated with RF (r = 0.445). In RA patients, a
significant association with the 721A allele of ICAM1 polymorphism (p = 0.04),
was found. In addition, the allele impact (G/A+A/A) of this polymorphism was
confirmed, (p = 0.038, OR = 2.3, C.I. 1.1–5.0). sVCAM-1 and sICAM-1 serum
levels reflected the clinical status in RA, independently of the ICAM1 and
VCAM1 polymorphism. However, the ICAM1 721A allele could be a genetic marker to
RA susceptibility.
