Affiliations: BiomeD Research & Publishing Group | Institute of Pathophysiology, Faculty of Medicine,
Comenius University, Bratislava, Slovak republic | Institute of Physiology, Faculty of Medicine, Comenius
University, Bratislava, Slovak republic | Institute of Medical Chemistry, Biochemistry and
Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava,
Slovak republic | Department of Molecular Biology, Faculty of Natural
Sciences, Comenius University, Bratislava, Slovak republic
Note: [] Corresponding author: Peter Celec, MD, Dipl. Ing., MSc, PhD,
MPH, Institute of Pathophysiology, Department of Molecular Biology, Comenius
University, Sasinkova 4, 811 08 Bratislava, Slovak republic. E-mail:
[email protected]
Abstract: Introduction: Salivary TBARS are a potential marker of oxidative
stress in the oral cavity. Previous studies have found increased levels of
salivary TBARS in various diseases. The aim of this study was to assess the
variability of salivary TBARS in both genders. Subjects & Methods: Saliva samples from thirty-eight
healthy volunteers (18F & 20M) were collected every day during 30 day
period. TBARS levels were measured spectrophotometrically using a
high-throughput 96-well plate method. Time series analysis was performed using
standard statistical methods. Results: Repeated measures ANOVA showed a significant
variation of salivary TBARS within day and subjects (p < 0.001). The
dynamics did not differ between genders. Intraindividual variability was very
high in both genders with coefficients of variation of more than 60%.
Interindividual variability was higher in men than in women (73% vs. 46%;
p < 0.01). Discussion: The relatively high intraindividual variability
indicates that the use of salivary TBARS will be limited to research on a
population level, although some informative value might be gained by repeated
samplings. Factors influencing the biological variability of salivary TBARS
should be identified in further studies.
