Matriptase and survivin expression associated with tumor progression and malignant potential in breast cancer of Chinese women: Tissue microarray analysis of immunostaining scores with clinicopathological parameters

Article type: Research Article

Authors: Tsai, Wen-Chiuan | Chu, Chi-Hong | Yu, Cheng-Pin | Sheu, Lai-Fa | Chen, Ann | Chiang, Hung | Jin, Jong-Shiaw

Affiliations: Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan | Division of General Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan | Taipei Institute of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan

Note: [] Corresponding author: Jong-Shiaw Jin, M.D., Ph.D., Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Cheng-Kung Road, Neihu 114, Taipei, Taiwan. Tel.: +886 2 87927155; Fax: +886 2 26913324; E-mail: [email protected]

Abstract: Objective: The aim of this study was to examine the expression of matriptase and survivin in breast carcinoma and correlate with clinicopathological parameters. Methods: Immunohistochemical analysis of matriptase and survivin were performed in tissue microarray slides of 290 cases, including 11 normal breast tissue; 27 fibrocystic disease; 17 fibroadenoma; 6 atypical ductal hyperplasia; 39 ductal carcinoma in situ, low grade (DCIS, low grade); 39 ductal carcinoma in situ, high grade (DCIS, high grade); 27 invasive ductal carcinoma, grade I (IDC, grade I); 78 invasive ductal carcinoma, grade II (IDC, grade II); and 46 invasive ductal carcinoma, grade III (IDC, grade III). Results: The average immunostaining scores of matriptase were 44.1 in normal breast tissue, 52.7 in fibrocystic disease, 76.5 in fibroadenoma, 81.7 in atypical ductal hyperplasia, 133.7 in low-grade DCIS, and 155.8 in high-grade DCIS. Among 151 breast IDC cases, the average immunostaining scores of matriptase were 172.7 in grade I, 211.7 in grade II, and 221.2 in grade III. Additionally, the average immunostaining scores of surviving also correlate with tumor grades and stages. Conclusions: Higher expressions of matriptase and survivin correlate significantly with clinicopathological parameters in breast cancer and the malignant potential in premalignant lesions. In addition, higher survivin expression had poorer prognosis of breast IDC cases.

Keywords: Atypical ductal hyperplasia, ductal carcinoma in situ, invasive ductal carcinoma, matriptase, survivin

Journal: Disease Markers, vol. 24, no. 2, pp. 89-99, 2008