Affiliations: Department of Biochemistry, All India Institute of
Medical Sciences, New Delhi-110029, India | Department of Neurology, All India Institute of
Medical Sciences, New Delhi-110029, India | Department of Biostatistics, All India Institute of
Medical Sciences, New Delhi-110029, India | Department of Cardiac Biochemistry, All India
Institute of Medical Sciences, New Delhi-110029, India | Department of Peadiatric Surgery, All India Institute
of Medical Sciences, New Delhi-110029, India
Note: [] Corresponding author: Dr. Kalpana Luthra, Associate Professor,
Room No. 3002, Department of Biochemistry, All India Institute of Medical
Sciences, New Delhi-110029, India. Tel.: +91 11 26588500 ex. 4483; Fax: +91 11
26588641; E-mail: [email protected]; [email protected]
Abstract: Purpose: To investigate the relationship of apolipoprotein E (apoE)
genotype, plasma levels of apoE and lipids in temporal lobe epilepsy (TLE)
patients in Asian Indians. Status of plasma levels of Apo E in epilepsy
patients has not been reported till date. Methods: ApoE gene polymorphism was analyzed in 58 patients with
temporal lobe epilepsy (TLE) and 57 age and sex approximated controls using
Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP).
Levels of plasma apoE and lipids were measured using ELISA and enzymatic kits
respectively. Results: The distribution of ApoE genotype in epilepsy patients and
controls was comparable. Higher levels of plasma ApoE were observed in TLE
patients as compared to controls (p=0.0001). Individuals with plasma levels of
apoE > 190 mg/L were at 20 times higher odds (95%CI=2.46–163.34,
p=0.005), while those with levels of apoE between 150–190 mg/L were at
4.9 times higher odds (95% CI=1.85–13.9, p=0.001), to develop TLE. Conclusions: We have observed for the first time, high levels of
plasma apoE in epilepsy patients. The findings of this case-control study
suggest that apolipoprotein E may play an important role in epilepsy.
Keywords: Apolipoprotein E, gene polymorphism, plasma apo E levels, temporal lobe epilepsy, asian Indians
Journal: Disease Markers, vol. 22, no. 5-6, pp. 335-342, 2006
