Article type: Research Article
Authors: Sánchez-González, V.J.; | Ortiz, G.G. | Gallegos-Arreola, P. | Macías-Islas, M.A. | Arias-Merino, E.D. | Loera-Castañeda, V. | Martínez-Cano, E. | Velázquez-Brizuela, I.E. | Rosales-Corral, S.A. | Curiel-Ortega, C.R. | Pacheco-Moisés, F. | García, J.J.
Affiliations: Laboratorio de Desarrollo-Envejecimiento, Enfermedades
Neurodegenerativas, División de Neurociencias, Centro de
Investigación Biomédica de Occidente (CIBO), Instituto Mexicano
del Seguro Social (IMSS), Guadalajara, Jalisco, México | Laboratorio de Biología Molecular,
División de Genética, CIBO-IMSS, Guadalajara, Jalisco,
México | Departamento de Neurología, Hospital de
Especialidades del Centro Médico Nacional de Occidente, IMSS,
Guadalajara, Jalisco, México, and Departamento de Neurociencias CUCS,
Universidad de Guadalajara | Departamento de Salud Pública, Centro
Universitario de Ciencias de la Salud, U de G. Guadalajara, Jalisco,
México | Departamento de Química, Centro Universitario
de Ciencias Exactas e Ingeniería, U de G. Guadalajara, Jalisco,
México | Departamento de Bioquímica, Instituto Nacional
de Cardiología, México
Note: [] Corresponding authors: Genaro G. Ortiz, MD., PhD. and Victor J.
Sánchez González, MD, Lab. Desarrollo-Envejecimiento,
Enfermedades Neurodegenerativas. CIBO-IMSS, Sierra Mojada 800 CP 44340,
Guadalajara, Jalisco, México. E-mail:
{genarogabriel,vjzahiiir}@yahoo.com
Abstract: Objective: To determine the β-amyloid precursor protein
(βAPP) isoforms ratio as a risk factor for Alzheimer's Disease and to
assess its relationship with demographic and genetic variables of the disease.
Methods: Blood samples from 26 patients fulfilling NINCDS-ADRDA
diagnostic criteria for AD and 46 healthy control subjects were collected for
Western blotting for βAPP. A ratio of βAPP isoforms, in optical
densities, between the upper band (130 Kd) and the lower bands (106–110
Kd) was obtained. Odds ratios were obtained to determine risk factor of this
component. Results: βAPP ratio on AD subjects was lower than that of
control subjects: 0.3662 ± 0.1891 vs. 0.6769 ± 0.1021 (mean
± SD, p<0.05). A low βAPP ratio (<0.6) showed an OR of
4.63 (95% CI 1.45–15.33). When onset of disease was taken into account, a
βAPP ratio on EOAD subjects of 0.3965 ± 0.1916 was found vs. 0.3445
± 0.1965 on LOAD subjects (p>0.05). Conclusions: Altered βAPP isoforms is a high risk factor for
Alzheimer's disease, although it has no influence on the time of onset of the
disease.
Keywords: β-Amyloid precursor protein, Alzheimer's Disease, risk factor
Journal: Disease Markers, vol. 22, no. 3, pp. 119-125, 2006
Received 29 May 2006
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Accepted 29 May 2006
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Published: 2006
