Affiliations: [a] Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, China | [b] Department of Biochemistry, Basic Medical School, Changchun Medical College, Changchun, Jilin, China | [c] Department of Hematology and Oncology, The First Bethune Hospital of Jilin University, Changchun, Jilin, China
Correspondence:
[*]
Corresponding author: Guixiang Tai, Department of Immunology, College of Basic Medical Sciences, Jilin University, 126 Xinmin Street, Changchun 130021, Jilin, China. E-mail:[email protected]
Note: [1] Juan Wang and Shufang Zhang contributed equally to this work.
Abstract: BACKGROUND: Mucin 1 (MUC1), as an oncogene, plays an important role
in the diagnosis of lung cancer. OBJECTIVE: To establish a double-antibody sandwich enzyme-linked
immune sorbent assay (ELISA) kit for the detection of serum MUC1 in lung
cancer patients. METHODS: Commercial mouse anti-human MUC1 monoclonal antibody and
rabbit anti-human MUC1 polyclonal antibody were used to construct a
double-antibody sandwich ELISA kit. The serum MUC1 levels in peripheral
blood of lung disease patients and healthy individuals were detected by the
double-antibody sandwich ELISA kit and CA15-3 kit, respectively. RESULTS: A double-antibody sandwich ELISA kit was successfully
constructed, and the sensitivity was up to 0.5 μ g/l. The cut-off value
for the serum MUC1 levels in the peripheral blood was 1.98 μ g/l, the
sensitivity was 62.5%, the specificity was 100% and the Youden index
was 0.6250 when detected by the double-antibody sandwich ELISA kit, while
the sensitivity was 18.75%, the specificity was 100% and the Youden
index was 0.1875 when detected by CA15-3 kit. CONCLUSION: The double-antibody sandwich ELISA kit is superior to
the CA15-3 kit in the detection of serum MUC1 in lung cancer patients,
suggesting an attractive applying of the double-antibody sandwich ELISA kit
in the early diagnosis of lung cancer.
Keywords: MUC1, anti-MUC1 polyclonal antibody, ELISA, CA15-3, lung cancer
