Affiliations: [a] Biochemistry Department, Assiut University, Assiut, Egypt | [b] Medical Biochemistry and Molecular Biology Department, Cairo University, Cairo, Egypt | [c] Tropical Medicine and Gastroenterology Department, Assiut University, Assiut, Egypt | [d] Internal Medicine Department, Assiut University, Assiut, Egypt
Correspondence:
[*]
Corresponding author: Dina Sabry, Medical Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mail:[email protected]
Abstract: AIMS: The study aimed to investigate the quantitative expression of
NANOG, p38 α , NCF2, ELF and TGF-β genes in patients with
colorectal adenocarcinoma, adenoma and normal colonic tissue and their
correlation with SIRT-1 protein level expression. METHOD: This
study enrolled one hundred sixty seven patients; group A: 87 patients with
colonoscopic findings of no adenoma or adenocarcinoma and group B: 80
patients with colorectal mass. Consecutive colonoscopic examinations were
conducted, and tissue samples were taken from the colonic lesions/masses.
Total RNA was isolated and mRNA expression level of NANOG, mitogen activated
p38α , Neutrophil Cytosol Factor 2 (NCF2), Embryonic Liver Fodrin
(ELF) and Transforming Growth Factor Beta (TGF-β) genes were
quantified by qRT-PCR. Sirt-1 protein expression level was assessed by
quantitative western blot. RESULTS: There were significantly high
level of mRNA transcripts expression of the genes studied in patients with
adenocarcinoma and adenoma compared with normal tissue (P value
< 0.01), NANOG, NCF2, ELF and TGF-β at a cut of > 0.314, > 0.392, 0.349 and 0.333 respectively showed sensitivity (96.5%,
98.8%, 95.3%, 98.8%) and specificity of (95.3%, 92.6%,
89.5%, 93.8%) respectively in diagnosing colonic adenocarcinoma.
Sirt-1 protein level was significantly highly expressed in colorectal
adenocarcinoma compared to normal and adenoma colonic tissue and positively
correlated with NANOG. CONCLUSION: Over expression of NANOG, p38α , NCF2, ELF and
TGF-β genes in both cases of adenocarcinoma and adenoma could have a
diagnostic value. SIRT-1 and NANOG are high correlated biological markers
for diagnosis and prognosis follow up in patients with adenocarcinoma.
