Higher circulating levels of chemokine CXCL10 in patients with breast cancer: Evaluation of the influences of tumor stage and chemokine gene polymorphism

Article type: Research Article

Authors: Jafarzadeh, A.^{a; b; *} | Fooladseresht, H.^c | Nemati, M.^b | Assadollahi, Z.^d | Sheikhi, A.^e | Ghaderi, A.^{c; f}

Affiliations: [a] Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran | [b] Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran | [c] Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | [d] Department of Epidemiology and Biostatistics, Rafsanjan University of Medical Sciences, Rafsanjan, Iran | [e] Department of Immunology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran | [f] Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Correspondence: [*] Corresponding author: Abdollah Jafarzadeh, Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Tel.: +98 34 3433 9042; Fax: +98 34 3433 9660; E-mail:[email protected]

Abstract: BACKGROUND: The CXCL10 receptor, CXCR3, is preferentially expressed on Th1 and NK cells. Therefore, CXCL10 acts as a chemoattractant for these cells. OBJECTIVE: The aim was to evaluate the CXCL10 levels and a single nucleotide polymorphism (SNP), rs4508917, in chemokine gene, in patients with breast cancer (BC). METHODS: A total of 200 subjects including 100 women with BC and 100 healthy women were enrolled into study. The serum CXCL10 levels were measured by ELISA and the SNP rs4508917 was determined by polymerase chain reaction-restriction length polymorphism (PCR-RFLP). RESULTS: The CXCL10 levels were significantly higher in patients than control group (P< 0.0001). There was also significant difference between tumor stages regarding the CXCL10 levels (P< 0.0001). The frequencies of GG genotype and G allele at rs4508917 were significantly higher in patients than controls (P< 0.0001). The CXCL10 levels were higher in patients with GG genotype whereas they were lower in healthy subjects having GG genotype as compared with those having AA genotype at rs4508917 (P< 0.001). CONCLUSION: Higher CXCL10 levels in patients with BC represent that the chemokine may contributes in tumor development. The rs4508917 may play a role in the susceptibility to BC. Different association was also observed between rs4508917 and CXCL10 levels in patients with BC and healthy subjects.

Keywords: Breast cancer, chemokine, CXCL10, gene polymorphism, rs4508917

DOI: 10.3233/CBM-160596

Journal: Cancer Biomarkers, vol. 16, no. 4, pp. 545-554, 2016