Affiliations: [a] Department of Oncology, Shunde TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Foshan, Guangdong, China | [b] Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China
Correspondence:
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Corresponding author: Jing-Bin Huang, Department of Oncology, Shunde TCM Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Foshan, Guangdong 528333, China. Tel./Fax: +86 757 22322743; E-mail:huang7373@163
Abstract: Meningioma is one of the common brain tumors in adults.
It had been shown that the allopregnanolone biosynthesis was associated with
tumorigenesis and PK11195, the translocator protein 18 KDa (TSPO) antagonist,
had the effects of the allopregnanolone biosynthesis. However, little is
known about the association between the effects of PK11195 on meningioma and
the allopregnanolone biosynthesis. To evaluate this, the meningioma cell
line IOMM-LEE was applied. Cell viability and proliferation were determined
by CCK-8 assay. The IC50 of PK11195 on the IOMM-LEE was 1.505 ± 0.08 nM.
The cell viability and proliferation of AC-5216 (TSPO selective ligand, 2
and 4 nM) was blocked by PK11195 (1.5 nM). Further, we evaluated the role of
allopregnanolone biosynthesis in the effects of TSPO on meningioma.
Enzyme-Linked ImmunoSorbent Assay (ELISA) was used in the measurement of the
allopregnanolone level. It showed that the allopregnanolone level was
increased by AC-5216 (2 and 4 nM) and the increase was reversed by PK11195
(1.5 nM). Collectedly, it firstly indicated that the effects of PK11195 on
meningioma were relevant to the decrease of allopregnanolone biosynthesis,
which was mediated by TSPO.
