Affiliations: Department of Vascular Surgery, the First Hospital of Jilin University, Changchun, Jilin, China
Correspondence:
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Corresponding author: Xi-Wei Sun, Department of Vascular Surgery, the First Hospital of Jilin University, Xinmin Street No.71, Chaoyang District, Changchun 130021, Jilin, China. Tel.: +86 18237372897; E-mail:[email protected]
Abstract: OBJECTIVE: This study was to explore the effects
of RNA interference mediated vascular endothelial growth factor (VEGF) gene
silencing on biological behavior of renal cell carcinoma (RCC), transplanted
renal tumor and angiogenesis in nude mice. METHODS: The specific siRNA sequence targeting VEGF were designed and synthesized to construct
hVEGF-siRNA plasmid which was transfected into RCC 786-O cells. Reverse
transcriptase-polymerase chain reaction (RT-PCR) was used for the detection
of VEGF gene expression and western blot was adopted for the examination of VEGF
protein expression. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay was used to detect cell growth as well as cell migration
and invasion. The transplanted renal tumor models in nude mice were
established, and the growth condition of nude mice, and VEGF protein
expression in transplanted tumor slices and the microvessel density (MVD)
were detected. RESULTS: The expression level of VEGF mRNA in
VEGF-siRNA group was significant lower than that in the control group and
negative group, suggesting that establishment of plasmid specifically
inhibited the expression of VEGF gene The expression level of VEGF protein in
VEGF-siRNA group was significant lower than that in the control group and
negative group. VEGF gene silencing has the significant inhibition effects on
proliferation, migration and invasion of RCC 786-O cells. The tumor weight,
VEGF protein positive rate and MVD in VEGF-siRNA group were significant
lower than those in negative group and blank group. CONCLUSION: The VEGF gene silencing could inhibit the cell proliferation, migration and invasion
of RCC 786-O cells; inhibition of VEGF protein expression could prevent
transplanted RCC growth and tumor angiogenesis.
