Affiliations: [a] Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran | [b] Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | [c] Digestive Disease Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
Correspondence:
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Corresponding author: Hossein Mozdarani, Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Tel.: +98 2182883830; E-mail:[email protected]
Abstract: BACKGROUND: MicroRNAs can function as oncogenes and tumor suppressor genes in human
cancers. The expression of miR-107 is high in various types of solid tumors.
OBJECTIVE: The aim of this study was to evaluate the tumor and serum level of miR-107
and its correlation to HIF-1α expression in gastric cancer patients.
METHODS: Quantitative real-time PCR was used to analyze the expression of miR-107 and
HIF-1α in 36 pairs of fresh gastric cancer and matched adjacent
normal tissue specimens and the serum of these patients compared to age
matched controls.
RESULTS: The expression level of miR-107 was significantly higher in tumor tissues
compared to adjacent normal tissue (p= 0.04). For serum, the expression
level of miR-107 was significantly higher in gastric cancer patients than in
age matched controls (p= 0.04). The correlation between tumor and serum
expression of miR-107 with tumor hypoxia was found to be significant (p≤ 0.001).
CONCLUSION: The overexpression of miR-107 in tumors and serum of gastric cancer patients and its correlation with HIF-1α expression in tumor tissues was
indicated that miR-107 may have a potential to use as a biomarker for
detection of gastric cancer patients and hypoxia in gastric cancer tumor.
