Affiliations: Plastic Surgery, The 1st Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China
Correspondence:
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Corresponding author: Huifan Liu, Plastic surgery, the 1st Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. E-mail:[email protected]
Abstract:
OBJECTIVE: The role of miR-365 in cancer cells seemed controversial
in previous studies. We thereby in this article aimed to define the role of
miR-365 in malignant melanoma (MM) pathogenesis. METHODS: We
detected miR-365 expression in malignant melanoma cell lines and then
investigated the effects of miR-365 on the metastasis and malignancy of
melanoma cells. The correlation between miR-365 level and NRP1 (neuropilin1)
was further investigated in clinical malignant melanoma specimens. RESULTS: MiR-365 was strongly down-regulated in malignant melanoma (MM)
tissues and cell lines, and its expression levels were associated with lymph
node metastasis and clinical stage, as well as overall survival and
replase-free survival of MM. We also found that ectopic expression of
miR-365 inhibited MM cell proliferation and MM metastasis in vitro and in vivo. We further
identified a novel mechanism of miR-365 to suppress MM growth and
metastasis. NRP1 was proved to be a direct target of miR-365, using
luciferase assay and western blot. NRP1 over-expression in miR-365 expressing
cells could rescue invasion and growth defects of miR-365. In addition,
miR-365 expression inversely correlated with NRP1 protein levels in MM. CONCLUSION: Our data suggest that miR-365 functions as a tumor
suppressor in MM development and progression, and holds promise as a
prognostic biomarker and potential therapeutic target for MM.
Keywords: Malignant melanoma (MM), neuropilin1 (NRP1), miR-365, invasion and metastasis, pathogenesis, target therapy, cancer biomarker
