Affiliations: [a] Department of Immunology and Cell Biology, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania | [b] Laboratory of Surgical Gastroenterology, Lithuanian University of Health Sciences, Kaunas, Lithuania | [c] Department of Biological DNA Modification, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania | [d] Department of Organic Chemistry, Kaunas University of Technology, Kaunas, Lithuania
Correspondence:
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Corresponding author: Arvydas Kanopka, Department of Immunology and Cell Biology, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania. Tel.: +370 5 2602124; Fax: +370 5 2602116; E-mail:[email protected]
Abstract:
BACKGROUND: Cell lines derived from human tumors have been
extensively used as experimental models of neoplastic disease. Although such
cell lines differ from both normal and cancerous tissue. OBJECTIVE: The data obtained used DNA and RNA microarray systems
does not give full information about protein expression levels in cells and
tissues. We present experimental evidence that splicing factor SRSF1, SRSF2,
U2AF35, U2AF65 and KHSRP expression levels in gastrointestinal tract (colon,
gastric and pancreatic) tumors differ compare to healthy tissues and in cell
lines, derived from corresponding organs. METHODS: Protein expression was analyzed using Western blots.
RT-PCR method was used for Fas and Rac splicing analysis. RESULTS: Obtained results provided a novel molecular
characterization of this important group of human cell lines and their
relationships to tumors in vivo. Expression levels of individual splicing factors
in tumors might serve as tumor markers. Not all experimental results
obtained from cell lines reflect changes that occur in tumors. Also Fas and
Rac, cancer associated genes, tumor associated sFas and Rac1b mRNA isoform
profiles in cell lines do not correspond to profiles that are observed in
tumors. CONCLUSIONS: Not all experimental results obtained in cell lines
reflect changes that occur in real tumors.
