Affiliations: [a] Department of Andrology, Jinan Second Maternal and Children’s Hospital, Jinan, Shandong, China | [b] Department of Obstetrics and Gynecology, Linyi Third People’s Hospital, Linyi, Shandong, China | [c] Department of Foreign Affairs, Liaocheng Third People’s Hospital, Liaocheng, Shandong, China | [d] Department of Urology Surgery, Qingdao Women and Children’s Hospital, Qingdao, Shandong, China
Correspondence:
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Corresponding author: Hongjian Liu, Department of Urology Surgery, Qingdao Women and Children’s Hospital, No. 217 Liaoyang West Road, North District, Qingdao, Shandong 266000, China. %****␣cbm-32-cbm203021_temp.tex␣Line␣25␣**** Tel.: +86 130 6124 8418; E-mail: [email protected].
Abstract: This paper aimed at investigating AS1 expression in prostate cancer (PCa) and its effects on the proliferation and invasion of prostate cancer cells (PCCs). The prostate tissues and the matched adjacent normal prostate tissues excised and preserved during radical prostatectomy in our hospital were collected. The LncRNA NCK1-AS1 expression was detected. PCa patients were followed up for three years to analyze their prognosis. The correlation of LncRNA NCK1-AS1 expression with clinicopathological features was analyzed. Human normal prostate cells and human PCCs were selected, in which LncRNA NCK1-AS1 expression was tested to screen and then transfect the cells. Cell proliferation, invasion and migration were detected. Cell cycles and apoptosis were analyzed. Compared with the adjacent normal tissues, LncRNA NCK1-AS1 was highly expressed in the prostate cancer tissues. Its expression was remarkably different in those with different stages of TNM and with lymphatic metastasis or not. The prognosis of patients with high LncRNA NCK1-AS1 expression was remarkably poorer than that of those with low expression. Compared with the human normal prostate cells, LncRNA NCK1-AS1 expression in the human PCCs remarkably rose, with the greatest difference in 22Rv1 cells. Compared with the Blank group, cell proliferation and the number of plate cloned cells remarkably reduced in the sh-NCK1-AS1 group. Additionally, in this group, the number of invasive and migratory cells remarkably reduced; the expression of invasion-related protein E-cadherin remarkably rose but that of MMP-2 remarkably reduced; cell cycles were arrested and the expression of cycle-related proteins (CDK4, CDK6, cyclin D1) remarkably reduced; the apoptotic rate and the expression of apoptosis-related protein Bax remarkably rose. LncRNA NCK1-AS1 is highly expressed in PCa, so its down-regulation can inhibit PCCs from proliferating and reduce the number of invasive cells.
