Affiliations: [a] Department of Vascular and Thyroid and Breast Surgery, Shanxian Central Hospital, Heze, Shandong, China | [b] Department of Oncology, Shanxian Central Hospital, Heze, Shandong, China
Correspondence:
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Corresponding author: Qizhu Shi, Department of Vascular and Thyroid and Breast Surgery, Shanxian Central Hospital, No. 1, Wenhua Road, Heze 274300, Shandong, China. Tel./Fax: +86 530 4558730; E-mail: [email protected].
Abstract: BACKGROUND: MicroRNAs (miRNAs) have been reported to serve as potential biomarkers in various cancer and play important roles in tumor progression. OBJECTIVE: The aim of this study was to investigate the prognostic significance and functional role of miR-383-5p in breast cancer. METHODS: The expression levels of miR-383-5p in breast cancer tissues and cell lines were measured using quantitative real-time PCR analysis. Kaplan-Meier curve and Cox regression analysis were used to explore the prognostic significance of miR-383-5p in breast cancer. The CCK-8 assay was used to assess cell proliferation ability. Transwell assays were used to assess cell migration and invasion abilities of breast cancer cells. RESULTS: The expression of miR-383-5p was significantly downregulated in breast cancer tissues and cell lines, compared with that in normal tissues and normal epithelial MCF-10A cells, respectively. The expression of miR-383-5p was associated with differentiation, lymph node metastasis, and TNM stage. Patients with low miR-383-5p expression had shorter overall survival than those with high miR-383-5p expression. Overexpression of miR-383-5p significantly inhibited cell proliferation, migration, and invasion, while downregulation of miR-383-5p promoted cell proliferation, migration, and invasion in vitro. LDHA was a direct target of miR-383-5p. CONCLUSIONS: Taken together, miR-383-5p was downregulated in breast cancer tissues and cell lines, and overexpression of miR-383-5p inhibited cell proliferation, migration, and invasion in breast cancer cells by targeting LDHA. Based on our findings, miR-383-5p may be a prognostic biomarker and therapeutic target for breast cancer.
Keywords: miR-383-5p, breast cancer, prognosis, proliferation, migration, invasion
