Low expression of KIF7 indicates poor prognosis in epithelial ovarian cancer

Article type: Research Article

Authors: Yao, Yaoshan^{a; b; 1} | Liu, Longyang^{c; 1} | He, Wenhua^d | Lin, Xian^e | Zhang, Xuan^b | Lin, Zhongqiu^f | Zeng, Zhaoyang^{c; *} | Guo, Suiqun^{a; *}

Affiliations: [a] Department of Obstetrics and Gynecology, Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, China | [b] Department of Gynecology Oncology, First People’s Hospital of Chenzhou, Chenzhou, Hunan, China | [c] Department of Gynecology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical Universtiy, Guangzhou, Guangdong, China | [d] Department of Cardiovascular Medicine, First People’s Hospital of Chenzhou, Hunan, China | [e] Department of Oncology, Fujian Provincial Cancer Hospital, Fuzhou, Fujian, China | [f] Department of Gynecology Oncology, The Memorial Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China

Correspondence: [*] Corresponding authors: Zhaoyang Zeng, Department of Gynecology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical Universtiy, Guangzhou, Guangdong 510315, China. Suiqun Guo, Department of Obstetrics and Gynecology, Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong 510630, China. E-mail: [email protected].

Note: [1] Co-first authors.

Abstract: The aim of the present study was to investigate kinesin family member 7 (KIF7) expression in epithelial ovarian cancer tissues (paraffin-embedded tissues and fresh) and to explore its expression, association with clinicopathological parameters and prognostic value in patients with epithelial ovarian cancer. A total of 113 paraffin-embedded tumor tissues of epithelial ovarian cancer patients diagnosed and operated at the memorial hospital of Sun Yat-sen University Between December 2009 and March 2017 and 41 paratumor tissues were collected for the present study and were assessed for KIF7 expression using immunohistochemistry. Furthermore, 22 fresh epithelial ovarian cancer tissues and their matched paratumor tissues were collected from the Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, between August 2013 and March 2019 and subjected to reverse-transcription quantitative PCR analysis to detect the mRNA expression of KIF7. The expression of KIF7 was lower in cancer tissues than in paratumor tissues, and KIF7 expression was associated with recurrence-free survival and overall survival in epithelial ovarian cancer patients. Furthermore, multivariate logistic regression analysis indicated that low KIF7 expression was an independent predictor of poor survival in patients with epithelial ovarian cancer. In conclusion, KIF7 has a tumor suppressor role in epithelial ovarian cancer and is a useful independent prognostic predictor. It may hold important value for the clinical diagnosis and treatment of epithelial ovarian cancer.

Keywords: kinesin family member 7, epithelial ovarian cancer, prognosis, immunohistochemistry, reverse-transcription quantitative PCR

DOI: 10.3233/CBM-190328

Journal: Cancer Biomarkers, vol. 26, no. 4, pp. 481-489, 2019