Affiliations: [a] Department of Oncology, Shengli Oilfield Central Hospital, Shandong, China | [b] Department of Magnetic Rsonance Emination, Shengli Oilfield Central Hospital, Shandong, China | [c] Xunshan Street Health Center, Rongcheng, Weihai, Shandong, China | [d] Department of Medical, Shengli Oilfield Central Hospital, Shandong, China
Correspondence:
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Corresponding author: Honglei Li, Department of Medical, Shengli Oilfield Central Hospital, No. 31 Jinan Road, Dongying District, Shandong 257000, China. Tel./Fax: +86 13695462508; E-mail: [email protected].
Abstract: Colorectal cancer (CRC) is a common cause of cancer-related deaths worldwide. MicroRNA-935 (miR-935) has been reported to be involved in several cancers. In the present study, we aimed to investigate the role of miR-935 in the progression of CRC. The expression levels of miR-935 in CRC tissues and cells were detected using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The prognostic significance of miR-935 was identified using Kaplan-Meier survival analysis and Cox regression assay. Cell proliferation capacity was detected by Cell Counting Kit-8 (CCK-8) assay. Cell migration and invasion capacities were determined by Transwell assay in CRC cells. Bioinformatics analysis and luciferase reporter assays were used to confirm the direct target of miR-935. The expression of miR-935 was increased in CRC tissues and cells. Overexpression of miR-935 was significantly associated with lymph node metastasis and TNM stage. Overexpression of miR-935 in patients predicted shorter overall survival compared to low miR-935 expression. The expression of miR-935 was indicated to be an independent prognostic factor for CRC patients. In addition, overexpression of miR-935 in CRC cells promoted cell proliferation, migration, and invasion, whereas inhibition of miR-935 suppressed cell proliferation, migration, and invasion. Bioinformatics and luciferase assays revealed that INPP4A is a direct target of miR-935. Our findings suggest that miR-935 functions as an oncogene and promotes CRC progression. INPP4A is a potential target of miR-935. And miR-935 may represent a prognostic biomarker and potential therapeutic strategy for CRC treatment.
