Affiliations: [a] Department of Ophthalmology, Jining No.1 People’s Hospital, Jining, Shandong, China | [b] Department of Clinical Laboratory, Rizhao Hospital of TCM, Rizhao, Shandong, China | [c] Department of Public Health, The People’s Hospital of Zhangqiu Area, Jinan, Shandong, China | [d] Department of Ward, The People’s Hospital of Zhangqiu Area, Jinan, Shandong, China | [e] Department of Hepatobiliary Gastrointestinal, The People’s Hospital of Zhangqiu Area, Jinan, Shandong, China | [f] Department of Ophthalmology, Yankuang New Journey General Hospital, Zoucheng, Shandong, China
Correspondence:
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Corresponding author: Li Zhao, Department of Ophthalmology, Yankuang New %****␣cbm-25-cbm182315_temp.tex␣Line␣25␣**** Journey General Hospital, No. 560 Kuangjian Dong Road, Zoucheng, Shandong 273500, China. Tel.: +86 537 5367064; E-mail: [email protected].
Abstract: BACKGROUND: Recently, the incidence and mortality of retinoblastoma (RB) have gradually increased. Many studies support the pivotal role of microRNAs (miRNAs) in the pathogenesis of RB. Alternation of microRNA-98 (miR-98) expression has been detected in several cancers, excluding RB. This study was designed to assess the regulatory mechanisms of miR-98 in human RB. METHODS: RT-qPCR and Western blot analysis were used to detect miR-98 and HMGA2 expression. The effects of miR-98 were explored using the CCK-8 and Transwell assays. Dual-luciferase reporter assay was performed to confirm the relationship between miR-98 and HMGA2. RESULTS: In RB, downregulation of miR-98 was identified. Upregulation of miR-98 inhibited proliferation, invasion and migration of RB cells. Further, HMGA2 was confirmed as a direct target gene of miR-98. And knockdown of HMGA2 suppressed the progression of RB. Moreover, upregulation of HMGA2 reversed the suppressive effects in the development of RB. In addition, miR-98 also showed suppressive effect on EMT and Wnt/β-catenin pathway. CONCLUSION: MiR-98 targets HMGA2 to act as a tumor suppressor in RB by mediating Wnt/β-catenin pathway.
